Hi Sequence Server Team!
Here’s an issue I’d like you to take a look at please.
In the following example, at first glance, it looks like my first hit has an evalue of 0, representing a highly significant hit.
However, it’s followed by a hit with evalue of ‘0.01’, a huge leap in evalue, which made me suspicious.
If you take a look at the details of the hits below, we see that the evalue reported as ‘0.00’ is infact 6.88E-4 rounded to 2 d.p. giving 0.00.
Could you please report evalues to enough decimal places or significant figures so that this rounding problem doesn’t happen.
To avoid the confusion, the 0.00 could be represented in any of these ways: 0.001 or 0.0007 or 6.88 x 10-4
Thanks so much!
Best wishes,
Simon
Number Sequences producing significant alignments Total score E value Length
- scaffold_574 43.51 0.00 62127
- scaffold_33 40.43 0.01 605089
- scaffold_32 40.05 0.01 626767
- scaffold_575 34.27 0.45 61993
- scaffold_1489 31.57 3.31 5924
- scaffold_2126 30.42 6.28 2535
scaffold_574 1 / 6
Hit length: 62127 Select | Sequence | FASTA
Score E value Identities Gaps Hit frame
43.51 (101) 6.88 × 10-04 50/193 (25.91) 34/193 (17.62) -2
Query 86 VLVVGSTGYIGRFVVKEMIKRGFNVIAVAREKSGIRGKNDKEETLKQL-----QGANVCF 140
VLV G GYIG V E++ GF + V N EE+LK++ + + F
Subject 49865 VLVTGGAGYIGSHTVLELVNEGFTAVVVDNL------DNSSEESLKRVSELTGKSDQIVF 49704
Query 141 SDVTELD–VLEKSIENLGFGVDVVVSCLASRNGGIKDSWKIDYEATKNSLVAGK—KF 195
V L+ LE + F + + L + +K K + +L+ K+
Subject 49703 RHVDLLNEEALEAVFKEFTFSACIHFAGLKAVGESVKLPLKYYHNNLTGTLILLNLLDKY 49524
Query 196 GAKHFVLLSAICV-----QKPLLE---------FQRAKLKFEAELMDLAEQQDSSFTYSI 241
G K+FV S+ V + P+LE + R KL E L DL+ Q+ + I
Subject 49523 GCKNFVFSSSATVYGLVKEMPVLEDAEIGATNPYGRTKLFIEEFLRDLSRAQE-EWNILI 49347
Query 242 VRPTAFFKSLGGQ 254
+R +F +G
Subject 49346 LR—YFNPVGAH 49317