Many thanks for placing this excellent resource in the public domain.
I would like to suggest an additional feature. Enable choice of a sequence file through a “Choose file…” interface with “start” and “stop” text-entry boxes defining the final query. We have a BLAST database comprised of a large collection of primer oligonucleotides and use it to locate possible appropriate oligos before running off and buying new ones. This feature would spare my colleagues loading a large genome in a text editor and locating, copying and pasting a region of interest.
I don’t think this would be very difficult to implement, inasmuch as one can almost do it now by loading the entire genome into the existing input window and then naming sequence limits in the “advanced parameters” box. I wouldn’t want computer-shy folks to have to do all that, though, when a much friendlier method could be used.